CDB15:0000382 CCN2 — ITGA5

Experimentally validated in Rat; Orthology-inferred in Human, Mouse, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

Connective tissue growth factor with a novel fibronectin binding site promotes cell adhesion and migration during rat oval cell activation.

Hepatology, 2008; PubMed, Rattus norvegicus Ccn2 — Rattus norvegicus Itga5

ABSTRACT: Oval cell activation, as part of the regenerative process after liver injury, involves considerable cell-matrix interaction. The matricellular protein, connective tissue growth factor (CTGF), has been shown to be critical for oval cell activation during liver regeneration following N-2-acetylaminofluorene/partial hepatectomy. To understand the mode of action of CTGF during this process, N-terminal CTGF was used as bait to screen a yeast two-hybrid complementary DNA library specific for regenerating livers with massive oval cell presence. Fibronectin (FN), a prominent component of hepatic extracellular matrix (ECM), was found to specifically bind to a new site on CTGF. In addition to module IV, this study showed that module I of CTGF was sufficient for binding to FN in both solid-phase in vitro binding assays and immunoprecipitation. Immunofluorescent staining revealed a dynamic ECM remodeling characterized by an FN-concentrated provisional matrix during oval cell-aided liver regeneration. Abundant CTGF protein was colocalized with FN in the provisional matrix. When expressed as recombinant proteins and immobilized on plastic surfaces, modules I and IV of CTGF were selectively adhesive to thymus cell antigen 1-positive (Thy1(+)) oval cells, stellate cells, and sinusoidal endothelial cells but not to hepatocytes. The adhesion of these two modules on Thy1(+) oval cells required heparan sulfate proteoglycan and integrin alpha(5)beta(1). Recombinant CTGF promoted an integrin alpha(5)beta(1)-dependent migration but not proliferation on Thy1(+) oval cells.

Basic Information on CCN2

Ligand Name: cellular communication network factor 2

Other Symbols: CTGF, IGFBP8

Ligand Location: secreted based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: CCN2

GeneCards: CCN2

HGNC Gene Group: Cellular communication network factors

Functional Annotations for CCN2

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for CCN2

CCN2 — ITGAM ・ CCN2 — ITGB2 ・ CCN2 — LRP1 ・ CCN2 — LRP6 ・ CCN2 — NTRK1 ・ CCN2 — FGFR2 ・ CCN2 — IGF2R ・ CCN2 — EGFR ・ CCN2 — FGFR1 ・ CCN2 — FGFR3 ・ CCN2 — ITGB1 ・ CCN2 — TGFBR1

Basic Information on ITGA5

Receptor Name: integrin subunit alpha 5

Other Symbols: FNRA, CD49e

Receptor Location: cell membrane based on perplexity, uniprot

HGNC Gene Symbol Report: ITGA5

GeneCards: ITGA5

HGNC Gene Group: CD molecules, Integrins

Functional Annotations for ITGA5

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ITGA5

ADAM15 — ITGA5 ・ ADAM17 — ITGA5 ・ ANGPT1 — ITGA5 ・ COL18A1 — ITGA5 ・ FBN1 — ITGA5 ・ FGA — ITGA5 ・ FN1 — ITGA5 ・ L1CAM — ITGA5 ・ SPP1 — ITGA5 ・ VTN — ITGA5 ・ ANGPTL2 — ITGA5 ・ CCN4 — ITGA5 ・ CXCL12 — ITGA5 ・ PLA2G2A — ITGA5 ・ SPON2 — ITGA5 ・ AGGF1 — ITGA5 ・ ANGPT2 — ITGA5 ・ ANGPTL4 — ITGA5 ・ CCN3 — ITGA5 ・ CD40LG — ITGA5 ・ COL4A3 — ITGA5 ・ IL1B — ITGA5 ・ MIA — ITGA5 ・ TGM2 — ITGA5 ・ THBS4 — ITGA5 ・ THY1 — ITGA5