CDB25:0004380 THBS4 — ITGA5

Experimentally validated in Human; Orthology-inferred in Mouse, Rat, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

THBS4 promotes HCC progression by regulating ITGB1 via FAK/PI3K/AKT pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2020; PubMed, Homo sapiens THBS4 — Homo sapiens ITGA5

ABSTRACT: Our research aims to identify the role of thrombospondin 4 (THBS4) in hepatocellular carcinoma (HCC). First, bioinformatic analysis was applied for detection the expression of THBS4 in HCC samples, and qRT-PCR and western blot were performed to explore the expression of THBS4 in HCC tissues and adjacent samples. Next, colony formation assay and cell viability assay were used to assess the function of THBS4 on HCC cells growth while transwell assay and scratch test were for metastasis. Meanwhile Xenograft tumor models were further conducted to verify the function of THBS4 in HCC. As for mechanism in deep, we investigated the influence of THBS4 on epithelial-mesenchymal transition (EMT) development and the interaction between THBS4 and integrin (ITG) family using multiple experiments, including western blot, immunofluorescence and immunoprecipitation (IP). As a result, our research discovered that the overexpression of THBS4 in both HCC patients' tissues and cell lines mediates HCC cells proliferation and metastasis in vitro and in vivo. In-depth, THBS4 regulated EMT progression and interacted with ITG family to modulate FAK/PI3K/AKT pathway. In conclusion, THBS4 as an oncogene interacts with integrinβ1 (ITGB1) to regulate HCC development via FAK/PI3K/AKT pathway.

Basic Information on THBS4

Ligand Name: thrombospondin 4

Other Symbols: N/A

Ligand Location: secreted based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: THBS4

GeneCards: THBS4

HGNC Gene Group: Thrombospondin family

Functional Annotations for THBS4

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for THBS4

THBS4 — ITGB1 ・ THBS4 — ITGB5 ・ THBS4 — NOTCH1

Basic Information on ITGA5

Receptor Name: integrin subunit alpha 5

Other Symbols: FNRA, CD49e

Receptor Location: cell membrane based on perplexity, uniprot

HGNC Gene Symbol Report: ITGA5

GeneCards: ITGA5

HGNC Gene Group: CD molecules, Integrins

Functional Annotations for ITGA5

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ITGA5

ADAM15 — ITGA5 ・ ADAM17 — ITGA5 ・ ANGPT1 — ITGA5 ・ COL18A1 — ITGA5 ・ CCN2 — ITGA5 ・ FBN1 — ITGA5 ・ FGA — ITGA5 ・ FN1 — ITGA5 ・ L1CAM — ITGA5 ・ SPP1 — ITGA5 ・ VTN — ITGA5 ・ ANGPTL2 — ITGA5 ・ CCN4 — ITGA5 ・ CXCL12 — ITGA5 ・ PLA2G2A — ITGA5 ・ SPON2 — ITGA5 ・ AGGF1 — ITGA5 ・ ANGPT2 — ITGA5 ・ ANGPTL4 — ITGA5 ・ CCN3 — ITGA5 ・ CD40LG — ITGA5 ・ COL4A3 — ITGA5 ・ IL1B — ITGA5 ・ MIA — ITGA5 ・ TGM2 — ITGA5 ・ THY1 — ITGA5