CDB25:0004436 VEGFA — CD44

Experimentally validated in Human, Mixed species; Orthology-inferred in Human, Mouse, Rat, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

Direct binding of hepatocyte growth factor and vascular endothelial growth factor to CD44v6.

Bioscience reports, 2015; PubMed, Homo sapiens VEGFA — Rattus norvegicus Cd44

ABSTRACT: CD44v6, a member of the CD44 family of transmembrane glycoproteins is a co-receptor for two receptor tyrosine kinases (RTKs), Met and VEGFR-2 (vascular endothelial growth factor receptor 2). CD44v6 is not only required for the activation of these RTKs but also for signalling. In order to understand the role of CD44v6 in Met and VEGFR-2 activation and signalling we tested whether CD44v6 binds to their ligands, HGF (hepatocyte growth factor) and VEGF (vascular endothelial growth factor), respectively. FACS analysis and cellular ELISA showed binding of HGF and VEGF only to cells expressing CD44v6. Direct binding of CD44v6 to HGF and VEGF was demonstrated in pull-down assays and the binding affinities were determined using MicroScale Thermophoresis, fluorescence correlation spectroscopy and fluorescence anisotropy. The binding affinity of CD44v6 to HGF is in the micromolar range in contrast with the high-affinity binding measured in the case of VEGF and CD44v6, which is in the nanomolar range. These data reveal a heparan sulfate-independent direct binding of CD44v6 to the ligands of Met and VEGFR-2 and suggest different roles of CD44v6 for these RTKs.

Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.

Arteriosclerosis, thrombosis, and vascular biology, 2017; PubMed, Homo sapiens VEGFA — Homo sapiens CD44

ABSTRACT: Vascular endothelial growth factor (VEGF), a major mediator of angiogenesis, exerts its proangiogenic action by binding to VEGFR2 (VEGF receptor 2), the activity of which is further modulated by VEGFR2 coreceptors such as neuropilins. However, whether VEGFR2 is regulated by additional coreceptors is not clear. To investigate whether SCUBE2 (signal peptide-CUB-EGF domain-containing protein 2), a peripheral membrane protein expressed in vascular endothelial cells (ECs) known to bind other signaling receptors, functions as a VEGFR2 coreceptor and to verify the role of SCUBE2 in the VEGF-induced angiogenesis.

Basic Information on VEGFA

Ligand Name: vascular endothelial growth factor A

Other Symbols: VEGF, VEGF-A, VPF

Ligand Location: secreted based on perplexity, uniprot

HGNC Gene Symbol Report: VEGFA

GeneCards: VEGFA

HGNC Gene Group: Receptor ligands: VEGF family

Functional Annotations for VEGFA

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for VEGFA

VEGFA — FLT1 ・ VEGFA — GPC1 ・ VEGFA — ITGA9 ・ VEGFA — ITGB1 ・ VEGFA — KDR ・ VEGFA — NRP1 ・ VEGFA — NRP2 ・ VEGFA — PDGFRB ・ VEGFA — PTPRZ1

Basic Information on CD44

Receptor Name: CD44 molecule (IN blood group)

Other Symbols: MIC4, MDU2, MDU3, IN, MC56, Pgp1, PGP-1, CD44R, HCELL, CSPG8, Hermes-1, CDw44, HUTCH-1, HUTCH-I, ECM-III, H-CAM

Receptor Location: cell membrane based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: CD44

GeneCards: CD44

HGNC Gene Group: Blood group antigens, CD molecules, Proteoglycans

Functional Annotations for CD44

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for CD44

COL14A1 — CD44 ・ COL1A1 — CD44 ・ COL1A2 — CD44 ・ FGF1 — CD44 ・ FGF2 — CD44 ・ FN1 — CD44 ・ HBEGF — CD44 ・ HGF — CD44 ・ MADCAM1 — CD44 ・ MMP7 — CD44 ・ MMP9 — CD44 ・ SPP1 — CD44 ・ VCAN — CD44 ・ VIM — CD44 ・ LGALS9 — CD44 ・ SELE — CD44 ・ BGN — CD44 ・ C1QA — CD44 ・ C1QB — CD44 ・ C1QC — CD44 ・ CD6 — CD44 ・ DMP1 — CD44 ・ GPNMB — CD44 ・ LAMA5 — CD44 ・ PRG4 — CD44 ・ SIGLEC15 — CD44