CDB25:0004445 VWF — ITGA4

Experimentally validated in Mixed species; Orthology-inferred in Human, Mouse, Rat, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

Tissue transglutaminase, coagulation factor XIII, and the pro-polypeptide of von Willebrand factor are all ligands for the integrins alpha 9beta 1 and alpha 4beta 1.

The Journal of biological chemistry, 2000; PubMed, Bos taurus VWF — Homo sapiens ITGA4

ABSTRACT: We previously reported that MOLT-3 human lymphocyte-like leukemia cells adhere to tissue-type transglutaminase (tTG) through the integrin alpha(4)beta(1). We now report that G-361 human melanoma cells also adhere to tTG, although they do not express alpha(4)beta(1). G-361 cells utilize two additional integrins, alpha(9)beta(1) and alpha(5)beta(1) to adhere to tTG. Furthermore, blood coagulation factor XIII (FXIII), another member of the transglutaminase family that is highly homologous to tTG, and propolypeptide of von Willebrand factor (pp-vWF) also promoted cell adhesion through alpha(9)beta(1) or alpha(4)beta(1) in G-361 or MOLT-3 cells, respectively. In the case of pp-vWF, alpha(9)beta(1) and alpha(4)beta(1) both bind to the same site, comprised of 15 amino acid residues and designated T2-15. Moreover, SW480 human colon cancer cells stably transfected to express alpha(9)beta(1), but not mock transfectants, adhered to tTG, FXIII, pp-vWF, and T2-15/bovine serum albumin conjugate. These data identify tTG, FXIII, and pp-vWF as shared ligands for the integrins alpha(9)beta(1) and alpha(4)beta(1). This report is the first to unambiguously show that these two integrins share the same cell adhesion site within one protein and provides strong support for classifying alpha(9)beta(1-) and alpha(4)-integrins as functionally related members of an integrin subfamily.

Basic Information on VWF

Ligand Name: von Willebrand factor

Other Symbols: F8VWF

Ligand Location: secreted based on perplexity, uniprot

HGNC Gene Symbol Report: VWF

GeneCards: VWF

HGNC Gene Group: Receptor ligands

Functional Annotations for VWF

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for VWF

VWF — GP1BA ・ VWF — ITGA2B ・ VWF — SELP ・ VWF — CLEC4M ・ VWF — GP9 ・ VWF — ITGA9 ・ VWF — ITGAV ・ VWF — ITGB1 ・ VWF — ITGB3 ・ VWF — LRP1 ・ VWF — SCARA5 ・ VWF — STAB2

Basic Information on ITGA4

Receptor Name: integrin subunit alpha 4

Other Symbols: CD49D, CD49d

Receptor Location: cell membrane based on hpa, perplexity

HGNC Gene Symbol Report: ITGA4

GeneCards: ITGA4

HGNC Gene Group: CD molecules, Integrins

Functional Annotations for ITGA4

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ITGA4

ADAM28 — ITGA4 ・ CD14 — ITGA4 ・ F13A1 — ITGA4 ・ FN1 — ITGA4 ・ ICAM4 — ITGA4 ・ MADCAM1 — ITGA4 ・ MDK — ITGA4 ・ SPP1 — ITGA4 ・ TGM2 — ITGA4 ・ THBS1 — ITGA4 ・ THBS2 — ITGA4 ・ VCAM1 — ITGA4 ・ ADAM11 — ITGA4 ・ ADAM23 — ITGA4 ・ ADAM29 — ITGA4 ・ ADAM7 — ITGA4 ・ CXCL12 — ITGA4 ・ PLA2G2A — ITGA4 ・ SPON2 — ITGA4 ・ ADAM2 — ITGA4 ・ CD40LG — ITGA4 ・ CX3CL1 — ITGA4 ・ JAM2 — ITGA4 ・ MIA — ITGA4 ・ PLAUR — ITGA4 ・ SVEP1 — ITGA4