CDB25:0003443 CST3 — LRP2

Experimentally validated in Mixed species; Orthology-inferred in Human, Mouse, Rat, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

Megalin-mediated endocytosis of cystatin C in proximal tubule cells.

Biochemical and biophysical research communications, 2007; PubMed, Homo sapiens CST3 — Rattus norvegicus Lrp2

ABSTRACT: Serum levels of cystatin C, an endogenous cysteine proteinase inhibitor, are often used as an indicator of glomerular filtration rate. Although it is known that cystatin C is filtered by glomeruli and metabolized in proximal tubule cells (PTC), the precise molecular mechanism underlying this process is undetermined. Using quartz-crystal microbalance analyses, we demonstrate that cystatin C binds directly to megalin, an endocytic receptor in PTC, in a Ca(+)-dependent manner. We also find that cystatin C is endocytosed specifically via megalin in rat yolk sac epithelium-derived L2 cells which share a variety of characteristics with PTC. Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC.

Basic Information on CST3

Ligand Name: cystatin C

Other Symbols: N/A

Ligand Location: secreted based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: CST3

GeneCards: CST3

HGNC Gene Group: Cystatin superfamily

Functional Annotations for CST3

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for CST3

Basic Information on LRP2

Receptor Name: LDL receptor related protein 2

Other Symbols: gp330, DBS

Receptor Location: cell membrane based on perplexity, uniprot

HGNC Gene Symbol Report: LRP2

GeneCards: LRP2

HGNC Gene Group: Low density lipoprotein receptors

Functional Annotations for LRP2

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for LRP2

AGT — LRP2 ・ ALB — LRP2 ・ APOB — LRP2 ・ APOE — LRP2 ・ INS — LRP2 ・ LCN2 — LRP2 ・ LEP — LRP2 ・ LPA — LRP2 ・ LPL — LRP2 ・ LRPAP1 — LRP2 ・ PLAU — LRP2 ・ SCGB1A1 — LRP2 ・ SERPINE1 — LRP2 ・ SHH — LRP2 ・ TCN2 — LRP2 ・ TG — LRP2 ・ APOM — LRP2 ・ APP — LRP2 ・ CLU — LRP2 ・ CRIPTO — LRP2 ・ GC — LRP2 ・ LTF — LRP2