CDB25:0004324 SPP1 — ITGA8

Experimentally validated in Mouse; Orthology-inferred in Human, Rat, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

Fibroblast-Secreted Phosphoprotein 1 Mediates Extracellular Matrix Deposition and Inhibits Smooth Muscle Cell Contractility in Marfan Syndrome Aortic Aneurysm.

Journal of cardiovascular translational research, 2022; PubMed, Mus Musculus Spp1 — Mus Musculus Itga8

ABSTRACT: Fibrillin 1 (Fbn1) mutation causes Marfan syndrome (MFS) with thoracic aortic aneurysm (TAA) as the main complication. The mechanisms for extracellular matrix (ECM) homeostasis disruption in MFS TAA are unclear. Here, we found ECM-related gene secreted phosphoprotein 1 (Spp1) increased in Fbn1C1041G/+ mice using transcriptome sequencing and a distinct fibroblast subcluster with Spp1 as the strongest marker was identified with analysis of the MFS mouse aortic single-cell sequencing dataset. Immunostaining confirmed elevated Spp1 in adventitial fibroblasts, and Spp1 might regulate fibroblast and smooth muscle cell (SMC) communication primarily through Itga8/Itgb1. Then, we observed Spp1 reduced contractile genes Acta2 and Tagln expression in SMCs and increased collagen expression in fibroblasts, which might contribute to TAA development. Finally, we also found elevated SPP1 plasma level was associated with an increased risk of TAA in patients. Therefore, SPP1 may serve as a biomarker and therapeutic target for TAA.

Basic Information on SPP1

Ligand Name: secreted phosphoprotein 1

Other Symbols: BNSP, OPN, BSPI, ETA-1, lnc-PKD2-2-3

Ligand Location: secreted based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: SPP1

GeneCards: SPP1

HGNC Gene Group: Secretory calcium-binding phosphoprotein family, Receptor ligands

Functional Annotations for SPP1

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for SPP1

SPP1 — CD44 ・ SPP1 — ITGA4 ・ SPP1 — ITGA5 ・ SPP1 — ITGA9 ・ SPP1 — ITGAV ・ SPP1 — ITGB1 ・ SPP1 — ITGB3 ・ SPP1 — ITGB5 ・ SPP1 — ITGB6

Basic Information on ITGA8

Receptor Name: integrin subunit alpha 8

Other Symbols: N/A

Receptor Location: cell membrane based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: ITGA8

GeneCards: ITGA8

HGNC Gene Group: Integrins

Functional Annotations for ITGA8

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ITGA8

FN1 — ITGA8 ・ NPNT — ITGA8 ・ TNC — ITGA8 ・ VTN — ITGA8 ・ MFGE8 — ITGA8 ・ TGFB1 — ITGA8 ・ TGFB3 — ITGA8