CDB20:0002808 LGALS1 — CD69

Experimentally validated in Human; Orthology-inferred in Mouse, Rat, Frog, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

The leukocyte activation receptor CD69 controls T cell differentiation through its interaction with galectin-1.

Molecular and cellular biology, 2014; PubMed, Homo sapiens LGALS1 — Homo sapiens CD69

ABSTRACT: CD69 is involved in immune cell homeostasis, regulating the T cell-mediated immune response through the control of Th17 cell differentiation. However, natural ligands for CD69 have not yet been described. Using recombinant fusion proteins containing the extracellular domain of CD69, we have detected the presence of a ligand(s) for CD69 on human dendritic cells (DCs). Pulldown followed by mass spectrometry analyses of CD69-binding moieties on DCs identified galectin-1 as a CD69 counterreceptor. Surface plasmon resonance and anti-CD69 blocking analyses demonstrated a direct and specific interaction between CD69 and galectin-1 that was carbohydrate dependent. Functional assays with both human and mouse T cells demonstrated the role of CD69 in the negative effect of galectin-1 on Th17 differentiation. Our findings identify CD69 and galectin-1 to be a novel regulatory receptor-ligand pair that modulates Th17 effector cell differentiation and function.

Basic Information on LGALS1

Ligand Name: galectin 1

Other Symbols: GBP

Ligand Location: secreted based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: LGALS1

GeneCards: LGALS1

HGNC Gene Group: Receptor ligands, Galectins

Functional Annotations for LGALS1

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for LGALS1

LGALS1 — ITGB1 ・ LGALS1 — PTPRC ・ LGALS1 — CD7

Basic Information on CD69

Receptor Name: CD69 molecule

Other Symbols: CLEC2C

Receptor Location: cell membrane based on perplexity, uniprot

HGNC Gene Symbol Report: CD69

GeneCards: CD69

HGNC Gene Group: C-type lectin domain containing, CD molecules

Functional Annotations for CD69

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for CD69

MYL9 — CD69 ・ MYL12A — CD69 ・ MYL12B — CD69 ・ S100A8 — CD69 ・ S100A9 — CD69