CDB15:0000237 CCL2 — CCR3

Experimentally validated in Human; Orthology-inferred in Mouse, Rat, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

High level expression, activation, and antagonism of CC chemokine receptors CCR2 and CCR3 in Chinese hamster ovary cells.

Cytokine, 2004; PubMed, Homo sapiens CCL2 — Homo sapiens CCR3

ABSTRACT: The specificity of leukocyte trafficking in inflammation is controlled by the interactions of chemokines with chemokine receptors. Reliable structure-function studies of chemokine-receptor interactions would benefit from cell lines that express consistent high levels of chemokine receptors. We describe herein two new Chinese hamster ovary (CHO) cell lines in which the genes for chemokine receptors CCR2 and CCR3 have been incorporated into identical positions in the host genome. CCR2 is the primary receptor for the chemokine monocyte chemoattractant protein-1 (MCP-1) whereas CCR3 is the primary receptor for the chemokines eotaxin-1, eotaxin-2 and eotaxin-3. Both receptors are expressed at >5,000,000 copies per cell, substantially higher levels than in previous cell lines, and both are competent for binding and activation by the cognate chemokines for these receptors. Using these cell lines we confirm that eotaxin-1 and eotaxin-3 can act as an agonist and an antagonist, respectively, of CCR2. In addition, we show that eotaxin-2 is an antagonist of CCR2 and MCP-1 is an agonist of CCR3. Comparison of the chemokine sequences reveals several positions that are identical in MCP-1 and eotaxin-1 but different in eotaxin-2 and eotaxin-3, suggesting that these amino acids play a role in CCR2 activation.

Basic Information on CCL2

Ligand Name: C-C motif chemokine ligand 2

Other Symbols: SCYA2, MCP1, MCP-1, MCAF, SMC-CF, GDCF-2, HC11, MGC9434

Ligand Location: secreted based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: CCL2

GeneCards: CCL2

HGNC Gene Group: Receptor ligands: Chemokine ligands

Functional Annotations for CCL2

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for CCL2

CCL2 — ACKR2 ・ CCL2 — ACKR4 ・ CCL2 — CCR1 ・ CCL2 — CCR10 ・ CCL2 — CCR2 ・ CCL2 — CCR4 ・ CCL2 — CCR5 ・ CCL2 — ACKR1

Basic Information on CCR3

Receptor Name: C-C motif chemokine receptor 3

Other Symbols: CMKBR3, CC-CKR-3, CKR3, CD193

Receptor Location: cell membrane based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: CCR3

GeneCards: CCR3

HGNC Gene Group: 7TM proteins, CD molecules

Functional Annotations for CCR3

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for CCR3

CCL11 — CCR3 ・ CCL13 — CCR3 ・ CCL14 — CCR3 ・ CCL15 — CCR3 ・ CCL24 — CCR3 ・ CCL26 — CCR3 ・ CCL28 — CCR3 ・ CCL3L1 — CCR3 ・ CCL3 — CCR3 ・ CCL4 — CCR3 ・ CCL5 — CCR3 ・ CCL7 — CCR3 ・ CCL8 — CCR3 ・ CXCL10 — CCR3 ・ CXCL11 — CCR3 ・ CXCL9 — CCR3 ・ CCL18 — CCR3 ・ CCL4L1 — CCR3