CDB15:0000025 ADAM9 — ITGB5

Experimentally validated in Human; Orthology-inferred in Mouse, Rat, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

MDC-9 (ADAM-9/Meltrin gamma) functions as an adhesion molecule by binding the alpha(v)beta(5) integrin.

Biochemical and biophysical research communications, 2001; PubMed, Homo sapiens ADAM9 — Homo sapiens ITGB5

ABSTRACT: MDC-9 is a widely expressed member of the metalloproteinase/disintegrin/cysteine-rich protein family. The disintegrin domain of MDC-9 lacks an RGD motif but has recently been reported to bind the alpha(6)beta(1) integrin; however, it is unclear whether MDC-9 can bind other integrins. In the present study myeloma cells, but not lymphoblastoid cells, were shown to bind to immobilised, recombinantly expressed MDC-9 disintegrin domain (A9dis). Binding was divalent cation-dependent, being supported by Mn(2+) and Ca(2+). Adhesion of myeloma cells to A9dis was completely inhibited by an antibody to the alpha(v)beta(5) integrin but not by antibodies to other subunits. RGD-containing peptides had no effect on binding, suggesting that MDC-9 interacts with alpha(v)beta(5) in an RGD-independent manner. Flow cytometric analyses demonstrated that myeloma cells, but not lymphoblastoid cells, expressed alpha(v)beta(5) on the cell membrane. These data indicated that the disintegrin domain of MDC-9 can function as an adhesion molecule by interacting with an alpha(v)beta(5) integrin.

Basic Information on ADAM9

Ligand Name: ADAM metallopeptidase domain 9

Other Symbols: CORD9, MDC9, KIAA0021, MCMP, Mltng

Ligand Location: secreted based on hpa, perplexity, uniprot, cell membrane based on perplexity, uniprot

HGNC Gene Symbol Report: ADAM9

GeneCards: ADAM9

HGNC Gene Group: Metallopeptidases

Functional Annotations for ADAM9

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for ADAM9

ADAM9 — ITGA3 ・ ADAM9 — ITGA6 ・ ADAM9 — ITGAV ・ ADAM9 — ITGB1 ・ ADAM9 — ITGA1

Basic Information on ITGB5

Receptor Name: integrin subunit beta 5

Other Symbols: N/A

Receptor Location: cell membrane based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: ITGB5

GeneCards: ITGB5

HGNC Gene Group: Integrins

Functional Annotations for ITGB5

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ITGB5

CCN1 — ITGB5 ・ LTBP1 — ITGB5 ・ LTBP3 — ITGB5 ・ VTN — ITGB5 ・ FNDC5 — ITGB5 ・ SPP1 — ITGB5 ・ ANGPTL4 — ITGB5 ・ COL4A3 — ITGB5 ・ FCER2 — ITGB5 ・ IBSP — ITGB5 ・ ICAM4 — ITGB5 ・ MFGE8 — ITGB5 ・ POSTN — ITGB5 ・ THBS4 — ITGB5