CDB25:0004246 SDC1 — ADGRA2

Experimentally validated in Human; Orthology-inferred in Mouse, Rat, Frog, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

Pooled extracellular receptor-ligand interaction screening using CRISPR activation.

Genome biology, 2018; PubMed, Homo sapiens SDC1 — Homo sapiens ADGRA2

ABSTRACT: Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.

Basic Information on SDC1

Ligand Name: syndecan 1

Other Symbols: SDC, CD138, syndecan, SYND1

Ligand Location: secreted based on hpa, perplexity, uniprot, cell membrane based on perplexity

HGNC Gene Symbol Report: SDC1

GeneCards: SDC1

HGNC Gene Group: CD molecules, Proteoglycans

Functional Annotations for SDC1

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for SDC1

Basic Information on ADGRA2

Receptor Name: adhesion G protein-coupled receptor A2

Other Symbols: GPR124, TEM5, DKFZp434C211, DKFZp434J0911, KIAA1531, FLJ14390

Receptor Location: cell membrane based on hpa, perplexity, uniprot

HGNC Gene Symbol Report: ADGRA2

GeneCards: ADGRA2

HGNC Gene Group: 7TM proteins, Immunoglobulin superfamily domain containing

Functional Annotations for ADGRA2

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ADGRA2

SDC2 — ADGRA2 ・ WNT7A — ADGRA2 ・ WNT7B — ADGRA2