CDB15:0001132 NPPB — NPR3

ABSTRACT: To elucidate the ligand-receptor relationship of the natriuretic peptide system, which comprises at least three endogenous ligands, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), and three receptors, the ANP-A receptor or guanylate cyclase-A (GC-A), the ANP-B receptor or guanylate cyclase-B (GC-B), and the clearance receptor (C-receptor), we characterized the receptor preparations from human, bovine, and rat tissues and cultured cells with the aid of the binding assay, Northern blot technique, and the cGMP production method. Using these receptor preparations, we examined the binding affinities of ANP, BNP, and CNP for the C-receptor and their potencies for cGMP production via the ANP-A receptor (GC-A) and the ANP-B receptor (GC-B). These analyses revealed the presence of a marked species difference in the receptor selectivity of the natriuretic peptide family, especially among BNPs. Therefore, we investigated the receptor selectivity of the natriuretic peptide family using the homologous assay system with endogenous ligands and receptors of the same species. The rank order of binding affinity for the C-receptor was ANP greater than CNP greater than BNP in both humans and rats. The rank order of potency for cGMP production via the ANP-A receptor (GC-A) was ANP greater than or equal to BNP much greater than CNP, but that via the ANP-B receptor (GC-B) was CNP greater than ANP greater than or equal to BNP. These findings on the receptor selectivity of the natriuretic peptide family provide a new insight into the understanding of the physiological and clinical implications of the natriuretic peptide system.

ABSTRACT: Binding studies, affinity cross-linking and guanylate cyclase assays allowed a comparison of receptors with which the rat forms of atrial/A-type natriuretic peptide (rANP), brain/B-type natriuretic peptide (rBNP) and C-type natriuretic peptide (rCNP) interact in rat kidney cortex and lung. This work represents the first study in which the rat form of BNP (= rBNP-45/iso-rANP(1-45)) has been used as a radiolabelled tracer to further characterize its receptors in these tissues. In addition, these studies stress the use of the same species of natriuretic peptide and assay system, an important experimental des ign given that BNPs show species-specific differences in structure. rBNP-45 bound with lower affinity to rANP (99-126) receptors, namely guanylate cyclase-linked receptor(s) and C-receptor. No receptor which interacted with only rBNP-45 was detectable in lung and kidney cortex. Since rBNP-45 interacted preferentially with the C-receptor and was less potent than rANP(99-126) in stimulating glomerular guanylate cyclase, rBNP-45 may signal through another second messenger in addition to cyclic GMP. Work with truncated analogues of this hormone pinpointed regions of this peptide which may contribute to receptor binding affinity and guanylate cyclase activation. CNP-22 bound to only a subset of ANP receptors and was least effective in stimulating glomerular guanylate cyclase, suggesting a differential mode of action from ANP.