CDB15:0000344 COL4A1 — ITGA1

Experimentally validated in Human; Orthology-inferred in Mouse, Rat, Frog, Zebrafish, Chicken, Macaque, Pig, Dog, Cow, Chimp, Horse, Marmoset, Sheep

Title

Journal:; Year Published:

Abstract

The alpha 1 beta 1 integrin recognition site of the basement membrane collagen molecule [alpha 1(IV)]2 alpha 2(IV).

The EMBO journal, 1993; PubMed, Homo sapiens COL4A1 — Homo sapiens ITGA1

ABSTRACT: Cells interact with type IV collagen mainly via the integrins alpha 1 beta 1 and alpha 2 beta 1. A triple helical CNBr derived fragment CB3[IV], which contains the recognition sites for both integrins, was isolated from type IV collagen. Trypsin treatment of CB3[IV] gave rise to four smaller fragments, F1-F4, of which the smallest one, F4, contained the recognition site for alpha 1 beta 1. Further fragmentation of F4 by thermolysin treatment at 50 degrees C led to fragment TL1, which represents the C-terminal half of F4, and which was no longer able to interact with alpha 1 beta 1. Therefore the recognition site of alpha 1 beta 1 had to be located within the N-terminal half of F4, a position which was verified by electron micrographs of a crosslinked F2-alpha 1 beta 1 complex. Modification of the Arg and Asp residues, which abolished the binding activity of F4, led to the identification of Arg (461) within the alpha 2(IV) and Asp (461) within the alpha 1 (IV) chain as essential residues for the alpha 1 beta 1. The array of these two residues on the surface of the triple helix is discussed.

Basic Information on COL4A1

Ligand Name: collagen type IV alpha 1 chain

Other Symbols: N/A

Ligand Location: secreted based on perplexity, uniprot

HGNC Gene Symbol Report: COL4A1

GeneCards: COL4A1

HGNC Gene Group: Collagens

Functional Annotations for COL4A1

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Receptors for COL4A1

COL4A1 — ITGA2 ・ COL4A1 — ITGB1 ・ COL4A1 — ITGB8 ・ COL4A1 — ADGRG6 ・ COL4A1 — DDR1 ・ COL4A1 — DDR2

Basic Information on ITGA1

Receptor Name: integrin subunit alpha 1

Other Symbols: VLA1, CD49a

Receptor Location: cell membrane based on perplexity

HGNC Gene Symbol Report: ITGA1

GeneCards: ITGA1

HGNC Gene Group: CD molecules, Integrins

Functional Annotations for ITGA1

Gene Ontologies: PAN-GO, AmiGO 2

Expression Profile: Human Protein Atlas (RNA and protein), Human Cell Atlas (RNA), GEPIA cancer vs normal (RNA), GeneCards (RNA and protein)

Disease Annotations: MalaCards, OMIM, Open Targets Platform, DISEASES (text mining), AI summary (LLM)

Interactions with other Ligands for ITGA1

COL1A1 — ITGA1 ・ COL1A2 — ITGA1 ・ COL2A1 — ITGA1 ・ COL8A1 — ITGA1 ・ LAMA1 — ITGA1 ・ MATN1 — ITGA1 ・ SEMA7A — ITGA1 ・ ADAM9 — ITGA1 ・ COL3A1 — ITGA1 ・ THBS1 — ITGA1